A unique subseafloor microbiosphere in the Mariana Trench driven by episodic sedimentation.

Hadal trenches are characterized by enhanced and infrequent high-rate episodic sedimentation events that likely introduce not only labile organic carbon and key nutrients but also new microbes that significantly alter the subseafloor microbiosphere. Currently, the role of high-rate episodic sedimentation in controlling the composition of the hadal subseafloor microbiosphere is unknown. Here, analyses of carbon isotope composition in a ~ 750 cm long sediment core from the Challenger Deep revealed noncontinuous deposition, with anomalous 14C ages likely caused by seismically driven mass transport and the funneling effect of trench geomorphology. Microbial community composition and diverse enzyme activities in the upper ~ 27 cm differed from those at lower depths, probably due to sudden sediment deposition and differences in redox condition and organic matter availability. At lower depths, microbial population numbers, and composition remained relatively constant, except at some discrete depths with altered enzyme activity and microbial phyla abundance, possibly due to additional sudden sedimentation events of different magnitude. Evidence is provided of a unique role for high-rate episodic sedimentation events in controlling the subsurface microbiosphere in Earth's deepest ocean floor and highlight the need to perform thorough analysis over a large depth range to characterize hadal benthic populations. Such depositional processes are likely crucial in shaping deep-water geochemical environments and thereby the deep subseafloor biosphere. Supplementary Information: The online version contains supplementary material available at 10.1007/s42995-023-00212-y.

Pharmaceutical Residues in Edible Oysters along the Coasts of the East and South China Seas and Associated Health Risks to Humans and Wildlife.

The investigation of pharmaceuticals as emerging contaminants in marine biota has been insufficient. In this study, we examined the presence of 51 pharmaceuticals in edible oysters along the coasts of the East and South China Seas. Only nine pharmaceuticals were detected. The mean concentrations of all measured pharmaceuticals in oysters per site ranged from 0.804 to 15.1 ng g-1 of dry weight, with antihistamines being the most common. Brompheniramine and promethazine were identified in biota samples for the first time. Although no significant health risks to humans were identified through consumption of oysters, 100-1000 times higher health risks were observed for wildlife like water birds, seasnails, and starfishes. Specifically, sea snails that primarily feed on oysters were found to be at risk of exposure to ciprofloxacin, brompheniramine, and promethazine. These high risks could be attributed to the monotonous diet habits and relatively limited food sources of these organisms. Furthermore, taking chirality into consideration, chlorpheniramine in the oysters was enriched by the S-enantiomer, with a relative potency 1.1-1.3 times higher when chlorpheniramine was considered as a racemate. Overall, this study highlights the prevalence of antihistamines in seafood and underscores the importance of studying enantioselectivities of pharmaceuticals in health risk assessments.

Characteristics, distribution patterns and sources of atmospheric microplastics in the Bohai and Yellow Seas, China.

Although the prevalence of microplastics in the atmosphere has recently received considerable attention, there is little information available regarding the distribution of atmospheric microplastics over oceanic regions. In this study, during the summer and autumn months of 2022, we investigated atmospheric microplastics in four marine regions off the eastern coast of mainland China, namely, the southern, middle, and northern regions of the Yellow Sea, and the Bohai Sea. The abundance of atmospheric microplastics in these regions ranged from 1.65 to 16.80 items/100 m3 during summer and from 0.38 to 14.58 items/100 m3 during autumn, although we detected no significant differences in abundance among these regions. Polyamide, chlorinated polyethylene, and polyethylene terephthalate were identified as the main types of plastic polymer. On the basis of meteorological data and backward trajectory model analyses, we established that the atmospheric microplastics detected during summer were mainly derived from the adjacent marine atmosphere and that over the continental landmass in the vicinity of the sampling area, whereas microplastics detected during autumn appear to have originated mainly from the northeast of China. By influencing the settlement and migration of microplastics, meteorological factors, such as relative humidity and wind speed, were identified as potential factors determining the distribution and characteristics of the detected microplastics. Our findings in this study, revealing the origin and fate of marine atmospheric microplastics, make an important contribution to our current understanding of the distribution and transmission of microplastics within the surveyed region and potentially worldwide.

Bioaccumulation of pollutants in the green-lipped mussel Perna viridis: Assessing pollution abatement in Victoria Harbour and its adjacent aquaculture area, Hong Kong, and the minimal human health risks from mussel consumption.

The green-lipped mussel Perna viridis was utilised for pollution biomonitoring in Victoria Harbour and its adjacent aquaculture area in Hong Kong. P. viridis was collected from a reference site and redeployed at five study sites for five weeks during the dry and wet seasons of 2019. Our study found various polycyclic aromatic hydrocarbons (PAHs) and heavy metals in the mussel tissue, while polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) were not detected. P. viridis at the reference site generally displayed lower levels of pollutants. Comparing with previous research in the 1980s and 2000s, we observed substantial reduction in the tissue levels of PAHs, PCBs, OCPs and heavy metals in P. viridis. The human health risks associated with consuming these mussels were determined to be insignificant. Our findings imply that the Harbour Area Treatment Scheme has been effective in improving the water quality in Victoria Harbour and its adjacent aquaculture area.

Environmental heterogeneity of cold seep by biological trait analysis of marine nematodes at Site F cold seep in South China Sea.

Cold seeps provide high environmental heterogeneity for marine benthos. Site F is one of the active cold seeps in the South China Sea. In this study, free-living marine nematode communities were investigated at Site F and the adjacent deep-sea area. A total of 67 genera and 32 families were identified. The mean density at cold seep sites ranged from 13.6 to 181.8 ind./10 cm2, and that at the adjacent deep-sea sites ranged from 36.9 to 301.4 ind./10 cm2. At cold seep sites, the most dominant nematode genera were Desmoscolex, Pierrickia, Sabatieria, Halalaimus, and Dorylaimopsis while at deep-sea sites, the most dominant genera were Retrotheristus, Thalassomonhystera, Desmoscolex, Cobbia, and Halalaimus. Deposit feeders of nematodes were dominant at all sites. Results of biological trait analysis showed that there was high environmental heterogeneity for nematodes at Site F. Water depth, sediment organic matter content, and sand proportion had important influences on nematode communities.

Comparison of macrofaunal assemblages in temperate seagrass meadows and neighboring seabeds along the southeastern coast of Shandong Peninsula, China.

Comparative study of macrofaunal assemblages in seagrass meadows and neighboring seabeds along the southeastern coast of Shandong Peninsula, China were performed. A total of 136 species were identified, including polychaetes (49 species), crustaceans (28), molluscs (58), and echinoderms (1). Species numbers of macrofauna in seagrass meadows and the neighboring seabeds were 52 and 65, respectively, whereas those in autumn were 90 and 56, respectively. Average macrofaunal abundances in spring in seagrass and neighboring seabeds were 2388.9 and 2516.7 ind./m2, respectively, whereas those in autumn were 11,689.0 and 1733.3 ind./m2, respectively. Ranges of species richness index, evenness index, and Shannon-Wiener index in seagrass meadows and the neighboring seabeds were 1.3-2.7, 0.7-0.9, 2.8-3.8, and 1.04-2.4, 0.5-0.9, 1.6-3.4 during spring, whereas those in autumn were 0.1-4.2, 0.3-0.8, 0.8-3.6 and 1.4-3.5, 0.5-0.9, 2.2-4.5. Bottom water temperature, salinity, sediment chlorophyll a concentration, and water content were the most important environmental factors influencing macrofaunal assemblages.

Bioassessment of environmental quality based on taxonomic and functional traits of marine nematodes in the Bohai Sea, China.

Free-living marine nematodes are valuable biological indicators for different environmental disturbances. Their taxonomic composition and functional traits often respond to environmental changes. In this study, marine nematodes, collected from the Bohai sea on the northeastern coast of China in 2014, were investigated in terms of their taxonomic composition and functional traits. Furthermore, the environmental quality of the investigated area was assessed based on nematode metrics. The studied nematode community showed spatial variation in taxonomic and functional composition, in response to changes in environmental variables such as sediment chlorophyll-a, phaeophytin-a, organic matter content, silt-clay content etc. Overall, high percentage of tolerant marine nematodes species or colonizers predominated, suggesting a disturbed environmental condition of the study area. Further results from the environmental quality assessment based on nematode metrics indicated a moderate quality status at the most investigated stations.

Varying abundance of microplastics in tissues associates with different foraging strategies of coastal shorebirds in the Yellow Sea.

With the wide application of plastic products, microplastics are now ubiquitous in coastal wetlands, representing a serious threat to the health of coastal organisms. In East Asia, millions of migratory shorebirds depend on the tidal flats of Yellow Sea in China, and they have experienced rapid populations declines due at least partially to the environmental pollution. However, our understanding about the specific exposures and hazards of microplastics, and the factors affecting the bioavailability of microplastics to different shorebird species remains limited, which hinders our ability to address the potential detrimental effects of microplastic accumulation to these fast-disappearing birds. Therefore, this study aims to assess the risk of microplastic exposure in shorebirds, determine the enrichment of microplastics in different tissues, and establish the relationship between shorebirds' foraging strategies and microplastic intake. We extracted and identified microplastics in different tissues sample from the carcasses of 13 individuals in four shorebird species, and measure the abundance, color, size, and roughness of all microplastics found. Microplastics were found in all species except one red-necked stint (Calidris ruficollis). Polyethylene, silicone, polypropylene, and polyurethane were the main polymers identified in shorebirds. Microplastics found in shorebirds that use mixed tactile and visual foraging strategy were smaller, less rough, and low in color diversity, compared to those found in shorebirds that forage predominately using visual cues. In addition, ingested microplastics were disproportionately enriched in different tissues; in particular, the abundance and size of microplastics in the digestive tract were significantly higher than those in the pectoral muscles. Understanding the stress of microplastics posed to coastal shorebirds is critical to facilitate more effective and targeted measurements in coastal pollution control.

Functional diversity of macrofaunal assemblages as indicators to assess heavy metal pollution in the Bohai Sea, China.

Functional diversity of macrofaunal assemblages can reflect the composition and differences of functional traits, indicating their response to various contaminants, especially heavy metal pollution. We explored the effects of environment variables over gradients of heavy metal pollution on macrofaunal assemblages, using biological traits analysis, generalized linear model (GLM), AZTI marine biotic index (AMBI), and various biodiversity indexes. The RLQ (co-inertia analysis) and fourth-corner approaches were used to investigate the specific response of functional traits to heavy metal pollution. Most sites were environmentally degraded by heavy metal pollution and macrofaunal body size had a miniaturization trend. There was a significant correlation between functional diversity indexes and AMBI. The RLQ and fourth-corner analysis and GLM models showed that heavy metal and natural environmental gradients had a profound effect on functional diversity. The functional divergence and dispersion indexes, along with the abundance of some specific species, were appropriate indexes for heavy metal pollution.

Transcriptomic response of intertidal brittle star Ophiothrix exigua to seasonal variation.

Adaptation to seasonal change is essential for survival, and is especially critical for organisms living in physically harsh environments. Brittle stars (Ophiothrix), known as a keystone species, inhabiting the intertidal rocky ecosystem are affected by multiple stressors, but molecular insights into their adaptation remain poorly studied. In the present study, transcriptomic responses of Ophiothrix exigua from the intertidal habitats of the North Pacific Ocean during summer and winter are reported. A total of 12,844 differentially expressed genes (DEGs) were identified. Of these, 7102 genes were up-regulated and 5742 genes were down-regulated in summer relative to winter. One hundred fifty-two key DEGs, including 31 up-regulated and 121 down-regulated genes, were categorized into three major subcategories and seven subclasses. The key DEGs included heat shock cognate protein 70 (HSC70), toll-like receptor-2 (TLR2), cAMP-dependent protein kinase catalytic subunit beta-like isoform X2 (PKA), serine/threonine-protein kinase mTOR (MTOR), and ras-related c3 botulinum toxin substrate 1 isoform X1 (RAC1). Glutathione peroxidase-like (GPX) and tubulin superfamily members (TUBA, TUBB) were consistent across seasons. The main defense-related pathways in brittle star were phagosome, apoptosis, and glutathione metabolism. These findings would greatly enhance our understanding of the genomic basis of environmental adaptation in intertidal invertebrates.

Bottom aquaculture can improve the basic trophic pathways and enhance the secondary production: Implications from benthic food web analysis.

To evaluate the impact of bottom aquaculture on benthic ecosystems, characteristics of benthic food web were studied using stable isotope techniques during four seasons in a Manila clam (Ruditapes philippinarum) bottom aquaculture area in a semi-enclosed bay, China. Results showed that although nitrogen stable isotope values of food sources (particulate organic matter and phytoplankton) had significant seasonal differences, there were no significant seasonal changes for benthic food web structure. Manila clam bottom aquaculture can enhance the secondary productivity and improve the basic trophic pathways by providing bio-deposits. Besides particulate organic matter and phytoplankton, Manila clam could feed on self-generated feces with high nitrogen stable isotope values, and benthic micro- and macroalga with high carbon stable isotope values. Secondary productivity of the stations with a high degree of aquaculture was higher than that of stations with a low degree. Bivalve bottom culture may have a positive impact on benthic ecosystem functioning.

Distribution and pollution risk assessment of heavy metals in the surface sediment of the intertidal zones of the Yellow River Estuary, China.

Eleven heavy metals (V, Cr, Mn, Co, Fe, Ni, Cu, Zn, As, Cd, and Pb) in surface sediment in the intertidal zones of the Yellow River Estuary, China were analyzed by inductively coupled plasma-mass spectrometry in summer and autumn 2019. Results indicated that the 11 heavy metals had the same sources. Sediment particle size and total organic matter were the main factors influencing the distribution of heavy metals. Most sites in the intertidal zones of the study area were not contaminated with V, Cr, Fe, Co, Zn, Pb, Cu, As, Mn and Ni, while Cd was the priority pollutant. Heavy metal pollution risk in autumn was higher than that in summer, which may be due to hydrodynamic effects. The present study showed that the heavy metal concentrations in the study area had a temporal decreasing trend compared to previous studies, which may be due to the reduction in human activities.

Distribution and potential sources of microplastics in sediments in remote lakes of Tibet, China.

The prevalence of microplastics in water bodies such as oceans and rivers has received considerable attention in recent years. The present study contributes to this research effort by assessing microplastics in 12 remote lakes on the Tibetan Plateau, China. Despite the limited extent of human activities, at least 17 items and up to 2644 items of microplastics were found per kg of dried sediments collected from the lakes in Tibet. These values were considered high compared to the levels of microplastics reported in other lake areas worldwide. Our results showed that the most prevailing types of microplastics in the sediments were black or transparent fibers in the size range of 0.05-0.5 mm, which were mainly identified to be polyamide and polyethylene terephthalate using Fourier-transform infrared microspectroscopy. The number of microplastics found appeared to be higher in sediments with a higher silt and clay content. Atmospheric long-range transport, glacial meltwater and surface runoff represent potential pathways to carry microplastics from elsewhere to the remote lakes in Tibet. This study shall be of great significance in understanding the transport and distribution of microplastics in the environment at regional or global scale.

Patterns of species and functional diversity of macrofaunal assemblages and the bioassessment of benthic ecological quality status in the southern Yellow Sea.

Macrofauna are useful indicators of benthic ecological quality status, which were studied in summer and autumn of 2020 in the southern Yellow Sea, China. A total of 134 species were identified, and polychaetes represented the most dominant group. The dominant species across both seasons were Ophiura sarsii vadicola, Trigonothracia jinxingae, Heteromastus filiforms, Ninoё palmata, Thyasira tokunagai, Sigambra hanaokai, Ehlersileanira incisa hwanghaiensis, and Portlandia japonica. The average values of macrofaunal abundance and biomass were 386.68 ind./m2 and 28.21 g/m2, respectively. Species diversity during the autumn was slightly higher than that in the summer. Seasonal variations in functional diversity were also observed. BIOENV results indicated that water depth, bottom water temperature, and sediment phaeophorbide content was the optimal combination of parameters to explain macrofaunal assemblage variation. Bioassessment results, based on H', AMBI, and M-AMBI, showed that most sites in the southern Yellow Sea could be classed as moderate or good status.

Characteristics of macrofaunal assemblages and their relationships with environmental factors in a semi-enclosed bay.

Characteristics of macrofaunal assemblages and their relationships with environmental factors in a semi-enclosed bay were studied seasonally in China. A total of 251 species were identified, including polychaetes (104 species), crustaceans (73 species), mollusks (51 species), and other phyla (23 species). Mean values of macrofaunal abundance were 1210, 2483, 3209, 3600 ind./m2 while those of biomass were 56.88, 176.15, 136.28, 265.55 g/m2, respectively. Ranges of species richness index, evenness index, Shannon-Wiener index were 1.54-8.16, 0.17-0.90, 0.69-4.78, respectively. The diversity indices were affected by bottom water salinity and pH. BIOENV analysis showed that water depth, phaeophorbide, and silt-clay proportion had important impacts on macrofaunal assemblages while abundance and biomass were affected by bottom water temperature, salinity, and water depth. Compared with historical data, macrofaunal species number, abundance and biomass showed a slight increasing trend, which may be due to the water quality improvement of this bay.

Spatial patterns and co-occurrence networks of microbial communities related to environmental heterogeneity in deep-sea surface sediments around Yap Trench, Western Pacific Ocean.

Microbial communities are a large component of abyssal and hadal benthic environments, especially in deep-sea areas like Yap Trench, they provide a continuous source of nutrients and energy in their unique ecosystems. However, due to sampling difficulties, these microbial communities are relatively understudied. In the summer of 2017, sediment samples were collected from 21 stations around Yap Trench in the Western Pacific Ocean (mostly in the West Caroline Basin), at depths ranging from 3156 to 7837 m. Sediment samples from deep water depths and shallow water depths differed in organic matter content, median grain size, silt-clay content, and biodiversity. The structure of the microbial communities in the surface sediments had distinct relationships with environmental factors and their co-occurrence networks exhibited a clear spatial pattern. In addition, for both prokaryotes or eukaryotes, a combination of variables including silt-clay content, organic matter content, median grain size, and depth had the greatest impact on community structure. It was notable that fungi played important roles in the co-occurrence networks of deep water depth sediment samples while bacteria dominated those of shallow water depth samples. The differences in structure and ecological niches in the different networks were due to differences in sediment texture and organic matter content. Since clay had a positive effect on the diversity of bacteria, it had an indirect positive effect on fungi, leading to differences in biodiversity among different groups. More organic matter meant more nutrients were available for the growth and reproduction of microbes, which led to fewer niche overlaps. This study conducted an extensive and systematic sequencing survey of surface sediments around Yap Trench in the Western Pacific Ocean, providing insight into microbial responses to environmental heterogeneity in deep-sea benthic ecosystems.

Distribution patterns of microplastics in various tissues of the Zhikong scallop (Chlamys farreri) and in the surrounding culture seawater.

Microplastics have adverse effects on marine organisms. However, there are limited data on microplastics distribution patterns in various tissues of marine organisms. Microplastics in seawater and nine types of tissues of the Zhikong scallop (Chlamys farreri) from three coastal aquaculture areas were analysed. The results showed that in seawater, microplastics were mainly fibrous. There were no significant differences in microplastics abundance among the three areas. The concentrations of microplastics in the scallop anus, intestinal tract and kidney were substantially higher than those in the other tissues. Microplastics abundance ranged from 8 to 13 particles per scallop. Microplastics colours were mainly black, transparent and blue, and there were no significant tissue distribution patterns. Microplastics size ranged from 5 µm to 1 mm. The microplastics abundance in the haemolymph was significantly correlated with those of the surrounding seawater. Additional study is necessary to determine the toxic effects of microplastics on marine organisms.

Deep-sea meiofauna assemblages with special reference to marine nematodes in the Caiwei Guyot and a Polymetallic Nodule Field in the Pacific Ocean.

Meiofauna particularly marine nematodes around the Caiwei Guyot in the northwest Pacific Ocean and a Polymetallic Nodule Field in the northeast Pacific Ocean were studied. Due to the geographic structure, the Caiwei Guyot and the Polymetallic Nodule Field had different environmental characteristics. Meiofaunal abundances around the Guyot area ranged from 9.18 to 25.59 ind./10 cm2, which were much lower than those in the Polymetallic Nodule Field. Marine nematode was the most dominant group. A total of 123 species, belonging to 74 genera and 29 families were found. Xyalidae (21.43%), Cyatholaimidae (9.82%), Linhomoeidae (8.03%) were the dominant families. The values of species number, Margalef's species richness and Shannon-Wiener diversity index ranged from 15 to 62, 4.75 to 12.84 and 2.58 to 3.93, respectively. The combination of water depth, silt-clay content and chlorophyll-a concentration can best explain the differences of nematode community. This study provides a baseline for deep-sea meiofauna distribution.

Functional diversity patterns of macrofauna in the adjacent waters of the Yangtze River Estuary.

Functional diversity can reflect the overall differences in functional traits and indicate their response to environmental disturbance. Macrofaunal feeding functional groups and functional diversity were studied in the adjacent waters of the Yangtze River Estuary. The detritivorous group had the highest species number, abundance and secondary productivity while the canivorous group had the highest biomass. Pearson correlation analysis showed that functional evenness was negatively correlated with species number and Shannon-Wiener index. Functional divergence was negatively correlated with Simpson dominance index. Functional dispersion was positively correlated with species evenness, Shannon-Wiener index and Simpson dominance index. High values of functional diversity mainly cooccurred with high species diversity, uniform trait distribution and small niche overlap, indicating weak interspecific competition and high resources utilization. The dominant species were miniaturized and their biological traits analysis indicated that the study area has been disturbed for long time, resulting in the decline of the benthic ecosystem stability.

Benthic ecological quality assessment of the Bohai Sea, China using marine biotic indices.

The coastal ecological environments are heavily affected by anthropogenic activities as well as global climate change. Macrofauna can be used as good indicators for benthic ecological quality status due to its relatively slow movement ability under disturbance and a variety of assessment approaches have been established based on the characteristics of macrofauna. The benthic ecological quality of the Bohai Sea, China, is increasingly threatened by anthropogenic activities. In order to accurately assess the benthic ecological quality status in this region, Shannon-Wiener diversity index, ABC curve, AMBI, M-AMBI and macrofaunal feeding evenness index were used. Results showed that the northern Laizhou Bay and Bohai Bay were under slight disturbance while Liaodong Bay, the central Bohai Sea and Bohai Strait were under moderate disturbance. Overall, the ecological quality indicated by the assessment results of AMBI, Shannon-Wiener diversity index and feeding evenness index were better than that by M-AMBI and the ABC curve.